Fig. 1
Expression of selected C19MC miRNAs in different cell lines. a A scheme displaying relative genomic locations of the C19MC and the miR-372 family miRNAs on human chromosome 19q13.41. MiRNAs in green and blue boxes harbor the AAGUGC seed sequence in the canonical (nts 2-7) or non-canonical positions, respectively (see Fig. 2a). The proposed exon sequences (Ex) of the clusters (Bortolin-Cavaille et al. [24]) are shown in short gray bars between the miRNAs; introns (Int) 18 and 20 shown carry two of multiple miRNA genes analyzed. The eight C19MC miRNAs selected for expression analysis in (b) and (c) below are shown in bold, with the expression A-C grouping designations established in (b) and (c) shown at the bottom of the miRNAs. b Expression of selected C19MC miRNAs, determined based on copy number per cell, in mesenchymal stem cells (MSCs). The MSCs included are: MSC-AT, WJ0706, ASC-INV and ASC. ASC IPSC and MH#1 are iPSCs derived from ASC-INV and ASC Lonza, respectively. H6 and H9 are human ESC cell lines. c C19MC miRNA expression in cancer cells. The cell lines used are: CRL-1790: normal colon cells, Hs799. PI: normal placenta cells, HCT15 and SK-CO-1: colorectal cancer cells; HepG2: hepatocellular carcinoma cells, MCF7: breast cancer cells; JEG3: choriocarcinoma cells. Ct values ≥ 35 was used as the cut-off threshold in the analysis