Fig. 2

ADD1, ADD2, and ADD3 can be sequestered in the nucleus by LMB. a NIH3T3 cells were treated with (+) or without (-) 20 nM LMB for 3 h, followed by extensive washing to remove LMB (wash-out) before fixation. The cells were fixed and stained for the ERK protein and nuclei. The representative images are shown. The distribution of ERK in the nucleus and cytoplasm was measured (n > 600). The values (mean ± SD) are from three experiments. b NIH3T3 cells were treated with (+) or without (-) 20 nM LMB for 3 h, followed by extensive washing to remove LMB (wash-out) before fixation. The cells were fixed and stained for the ADD1 and nuclei. The representative images are shown. The distribution of ADD1 in the nucleus and cytoplasm was measured (n > 600). The values (mean ± SD) are from three experiments. c NIH3T3 cells transiently expressing HA-ADD2 and HA-ADD3 were treated with (+) or without (-) 20 nM LMB for 3 h, followed by extensive washing to remove LMB before fixation. The cells were fixed and stained for the HA-ADD and nuclei. The distribution of the HA-ADD proteins in the nucleus and cytoplasm was measured (n > 600). The values (mean ± SD) are from three experiments