Fig. 5

LAT1 and system b^0,+ mRNA expression was determined by Taqman real-time PCR analysis in the rat cerebrum and in TR-BBB cells. Total RNA (2 μg) was reverse-transcribed and cDNA was amplified by real-time PCR. (+) and (−) represent the presence or absence of reverse transcriptase, respectively. The results were calculated using the comparative Ct (2^−ΔΔCt) method for relative quantification based on GAPDH mRNA expression and are shown as a fraction of relative LAT1 and system b^0,+ expression in rat cerebrum and TR-BBB cells. Each value represents the mean ± standard error (SE) of three determinations