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Table 2 Effect of L-amino acids on uptake of [14C]L-citrulline in TR-BBB cells

From: Characteristics of L-citrulline transport through blood-brain barrier in the brain capillary endothelial cell line (TR-BBB cells)

Substrate

Concentration

(mM)

Uptake of [14C]L-citrulline

(% of control)

Control

 

100 ± 1.3

L-Citrulline

2

36.0 ± 2.1***

L-Valine

2

31.5 ± 1.4***

L-Leucine

2

49.4 ± 5.8***

 

20

17.2 ± 3.9***

L-Phenylalanine

2

13.7 ± 1.8***

 

20

13.4 ± 1.4***

L-Glutamine

2

37.5 ± 1.3***

L-Alanine

2

73.0 ± 6.4**

L-Serine

2

79.7 ± 2.5*

L-Cysteine

2

48.4 ± 2.6***

L-Arginine

2

59.9 ± 6.6**

 

20

53.2 ± 4.4***

L-Lysine

2

74.5 ± 9.0*

 

20

66.78 ± 2.1*

L-Glutamate

2

102.8 ± 7.8

L-Aspatate

2

101.5 ± 8.6

  1. [14C]L-Citrulline uptake by TR-BBB cells was performed at pH7.4 and 37 °C for 5 min in the presence or absence of 2–20 mM L- amino acids. Each value represents the mean ± S.E.M. (n = 3–4). *p < 0.05, **p < 0.01, ***p < 0.001; significantly different from control