Fig. 1

Higher level of ROS in the ALDH2*2 knock-in mice compared to the wild type mice. a Four different tissues (tongue, lung, kidney and brain) were isolated from C57BL/6 wild type (WT) mice, homogenized and the whole cell lysates were used to measure ROS levels using a reactive oxygen species (ROS) detection kit (Cell BioLabs, STA-342). The fluorescence intensity of 2′,7′-dichlorodihydrofluorescein (DCF) was measured within 30 min using a BioTek FL-600 plate reader (BioTek Instruments, Winooski, Vt., USA) at 485 nm excitation and 530 nm emission wavelengths. Data were expressed in nM of DCF as calculated from the standard curves using the standards of the kit. Results represent the means and SEMs of experiments with 3 samples each. b ROS level was measured from whole cell lysates of four different tissues of ALDH2*2 knock-in mice (referred to as HET from here on). Results are expressed as mean ± SEM (*p < 0.05 vs. respective wild type control, t-test, n = 6 each)