Fig. 8

Serotonin immunoreactive nerve fibers and WGA-HRP labelled signals were detected in the proximal and distal stumps of AdPGIS-transduced OEC-transplanted spinal cords. a-c: Serotonin immunoreactivity in the longitudinal section of spinal cord. Bar = 50 μm. d & e: WGA-HRP labelled signals with TMB positive staining in the proximal and distal spinal cords, respectively, of animals grafted with AdPGIS-OEC using anterograde WGA-HRP tracing from motor sensory cortex. Bar = 100 μm. a and b are longitudinal sections of proximal cord. The dotted line indicate the separation between grey and white matter in panel a. Arrow indicate 5-HT(+) fibers. c shows the 5-HT(+) fibers in the gray matter of OEC-transplanted distal cords (longitudinal section). Inset: higher magnification of boxed inset in (c). d and e are transverse sections of T7 (proximal) and T12 (distal) stumps, respectively