Fig. 2
Genes encoding Nodal signalling components exhibited decreased or abolished expression in klf8 morphants. The majority of klf8-MO1atg or klf8-MO2atg -injected embryos showed decreased or abolished spaw (arrow) expression in the left lateral plate mesoderm (LPM) at the 18 s stage (a-f). The majority of klf8 morphants failed to express lft1 in the left diencephalon (arrowhead) or heart (asterisk) (g- i), lft2 in the left heart (asterisk) (j-l), and pitx2 in the left LPM (white arrow) (m-o) at the 22 s stage. Dorsal views of embryos are shown. A, absent; B, bilateral; D, decreased; L, left; R, right