Fig. 2
PA-6 dose dependently inhibits IK1 currents carried by homotypic wild-type (WT), V93I and D172N KIR2.1 channels measured in the inside out mode. a Steady state KIR2.1 current traces from WT, V93I and D172N KIR2.1 channel containing inside-out patches elicited by a voltage ramp protocol from −100 to 100 mV, under baseline conditions (C) and upon application of 10, 100 and 1000 nM of PA-6. Measurements were performed using symmetrical high potassium concentrations at both sides of the patch. b IC50 curves of PA-6 for the inward (at −80 mV) and outward (at +50 mV) KIR2.1 currents of WT (n = 10), V93I (n = 9) and D172N (n = 11) channel containing patches. Error bars indicate s.e.m