Fig. 4

The regulatory relationship between SNHG15 and miR-141. a The bioinformatics predicted binding sites of miR-141 on SNHG15. Luciferase reporter assay was performed in U2OS (b) and MG63 (c) cells cotransfected with pGL3-SNHG15-WT or pGL3-SNHG15-MUT and miR-141 or miR-control. d qRT-PCR was carried out to determine the expressions of miR-141 in U2OS cells transfected with si-SNHG15, si-control, si-SNHG15 + anti-miR-141, or si-SNHG15 + anti-miR-control. GAPDH was chosen as the internal control. e qRT-PCR was carried out to examine the expression of miR-141 in MG63 transfected with pcDNA-SNHG15, vector, pcDNA-SNHG15 + miR-141, or pcDNA-SNHG15 + miR-control. GAPDH was chosen as the internal control. *P < 0.05 vs. control group