Fig. 2
From: Leptin OB3 peptide suppresses leptin-induced signaling and progression in ovarian cancer cells
Effects of leptin and the OB3 peptide on expressions of proliferative and metastasis-related genes in ovarian cancer cells. SKOV-3 cells were seeded in 6-well plates and treated with different concentrations of the leptin peptide, OB3 peptide, or their combination for 8 or 24 h. Cells were harvested and total RNA or proteins were extracted. qPCR experiments were conducted to examine expression of proliferative genes (Cyclin D1, PCNA, c-Myc and ERα) (a) and metastasis-related genes (MMP-2 and MMP-9) (b). Western blotting experiments were performed to investigate the accumulation of cyclin D1, PCNA and ERα. (c) (Data are the mean ± SD, n = 6) (* p < 0.05, ** p < 0.01, *** p < 0.001 compared to the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the leptin (10 μM)-treated group)