Fig. 3
From: Leptin OB3 peptide suppresses leptin-induced signaling and progression in ovarian cancer cells
The role of the OB-R in the inhibitory effect of the OB3 peptide in ovarian cancer cells. Basal expression of OB-R in SKOV-3 and OVCAR-3 cells were examined by qPCR (a). SKOV-3 cells were transfected with an OB-R shRNA plasmid or a scrambled plasmid. Cells were treated with either leptin (10 nM), OB3 (100 μM) or their combination for 8 h. Cells were harvested, total RNA was extracted and qPCR experiments were performed to investigate expressions of OB-R (b), c-Myc (c), and Cyclin D1 (d). (Data are the mean ± SD, n = 3) ($$$ p < 0.001 compared with SKOV-3 cells; *** p < 0.001 compared to the control group (scrambled plasmid transfected and non-treatment); ### p < 0.001 compared to 10 nM leptin)