Fig. 2

The effect of Lu/BCAM on focus and colony formation of NIH3T3 and NIH-Lu cells. a NIH3T3 and NIH-Lu cell lines were cultured and seeded in the presence of laminin10/11 (0.2 μg/ml) for 14 days. The cells were fixed and followed by Giemsa staining to determine focus formation. b The foci were counted and quantitative data are shown. This experiment was repeated three times. _***: p < 0.0001. c Cells were cultured and seeded in 0.33% agar-containing medium with or without laminin10/11 treatment (0.2 μg/ml). The colonies were shown. d The size and number of colonies were measured at day 14. Colonies with a diameter greater than 3 μm were counted as positive. *: p < 0.05