Fig. 1

hPSC-based 3D brain organoid models with single or multiple, varying sizes and numbers of ventricular zone (VZ)-like regions. Examples of hPSC-derived 3D brain organoid models are divided into models that contain single rosette-like structure (left panel) or randomized sizes and numbers of neuroepithelial rosettes (right panel). Overlapping regions (box) interrupt the cortical plate layer formation and create difficulties with reproducibility and quantification of the cytoarchitecture of brain organoids