Table 1 PCR primers were used in this study
From: The cytotoxic mechanism of epigallocatechin gallate on proliferative HaCaT keratinocytes
Gene name | primer sequence (5’➔3′) | Replicon Size bp |
|---|---|---|
p53 | Forward: 5′-CTCTGACTGTACCACCATCCACTA-3′ Reverse: 5′-GAGTTCCAAGGCCTCATTCAGCTC-3′ | 373 |
p21 | Forward: 5′-CTGAGCCGCGACTGTGATGCG-3′ Reverse: 5′-GGTCTGCCGCCGTTTTCGACC-3′ | 345 |
p16 | Forward: 5′-GCAGCATGGAGCCTTCGGCT-3′ Reverse: 5′-TGCAGCACCACCAGCGTGTC-3′ | 274 |
cyclin D1 | Forward: 5′-ATGGAACACCAGCTCCTGTGCTGC-3′ Reverse: 5′-TCAGATGTCCACGTCCCGCACGTCGG-3′ | 885 |
DEC1 | Forward: 5′-GTACCCTGCCCACATGTACC-3′ Reverse: 5′-GCTTGGCCAGATACTGAAGC-3’ | 395 |
Zac1 | Forward: 5’-TCTCACCAGTGTGCTCACTGTGAG-3′ Reverse: 5′-GGTGAGGTGATCCTTGCGCCCAA-3’ | 417 |
GAPDH | Forward: 5’-CTTCATTGACCTCAACTAC-3′ Reverse: 5′-GCCATCCACAGTCTTCTG-3’ | 463 |