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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Sudden cardiac death: focus on the genetics of channelopathies and cardiomyopathies

Fig. 2

Expression levels and stability of caveolin-3 wild type and caveolin-3 V82I mutant. a Recombinant expression of caveolin-3 wild type (Cav-3 WT) and caveolin-3 V82I mutant (Cav-3 V82I) in BHK cells transiently transfected with plasmids expressing either Cav-3 WT or Cav-3 V82I. b Evaluation of protein stability. Transfected BHK cells were treated with the eukaryote protein synthesis inhibitor cycloheximide (10 μg/ml) for the indicated length of time. A representative blot is shown on the left of panel b. Levels of residual caveolin-3 at the indicated time points (% of time 0) for Cav-3 WT and Cav-3 V82I mutant are shown on the right. Data are representative of four independent experiments. *, P < 0.05 vs WT at the respective time point; **, P < 0.01 vs WT at the respective time point. The figure has been completely adopted from [22]

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