Standardized, systemic phenotypic analysis reveals kidney dysfunction as main alteration of Kctd1 I27N mutant mice

Table 4 Clinical chemical analysis of line Kctd1 ^I27N

Parameter	Heterozygous mutant males	Wild-type males	Heterozygous mutant females	Wild-type females	Genotype: P-value
Na (mmol/l)	157 ± 2	156 ± 2	156 ± 3	156 ± 2
K (mmol/l)	5.1 ± 0.3	4.6 ± 0.2	4.4 ± 0.2	4.1 ± 0.2	< 0.001
Ca (mmol/l)	2.5 ± 0.1	2.5 ± 0.1	2.6 ± 0.1	2.5 ± 0.1	< 0.001
Cl (mmol/l)	113.5 ± 1.7	114.8 ± 1.7	113.9 ± 1.5	117.1 ± 1.4	< 0.001
P_i (mmol/l)	2.2 ± 0.2	2.2 ± 0.3	2.1 ± 0.3	2.1 ± 0.3
Total protein (g/l)	51.3 ± 2.4	51.6 ± 1.9	50.7 ± 1.9	48.7 ± 1.6
Creatinine (μmol/l)	9.4 ± 1.5	7.8 ± 1.2	10.6 ± 0.7	9.5 ± 1.2	< 0.001
Urea (mmol/l)	18.2 ± 1.5	10.8 ± 0.8	16.9 ± 1.4	10.1 ± 1.1	< 0.001
Cholesterol (mmol/l)	4.1 ± 0.4	4.2 ± 0.4	3.0 ± 0.3	2.8 ± 0.3
Triglycerides (mmol/l)	4.1 ± 0.5	4.2 ± 1.2	3.4 ± 0.8	3.4 ± 1.0
ALT (U/l)	37 ± 4	39 ± 9	32 ± 3	32 ± 3
AST (U/l)	50 ± 6	54 ± 17	51 ± 7	51 ± 7
AP (U/l)	90 ± 5	97 ± 8	132 ± 12	135 ± 8	0.04
α-Amylase (U/l)	776 ± 162	652 ± 54.	695 ± 91	534 ± 39	< 0.001
Glucose (mmol/l)	10.5 ± 2.0	9.2 ± 1.6	10.2 ± 1.5	9.8 ± 1.2	P > 0.05 (0.06)
Albumin (g/l)	28.7 ± 1.3	28.3 ± 1.3	30.8 ± 1.3	29.4 ± 1	0.02

17-week-old mice were tested. Number per genotype and sex: n = 11–14. Data are presented as mean ± standard deviation. Significance vs. wild-type controls: Exact P values are indicated for P < 0.05
Creatinine plasma creatinine analyzed by the enzymatic method, ALT alanine aminotransferase, AST aspartate aminotransferase, AP alkaline phosphatase

ISSN: 1423-0127