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Table 6 Urine analysis of line Kctd1 I27N

From: Standardized, systemic phenotypic analysis reveals kidney dysfunction as main alteration of Kctd1 I27N mutant mice

Parameter Heterozygous mutant males Wild-type males Heterozygous mutant females Wild-type females Genotype:
P-value
Body weight (g) 36.0 ± 1.9 37.1 ± 1.5 36.4 ± 3.3 37.5 ± 3.9  
Water ad libitum
Water intake (ml/day) 3.8 ± 0.8 3.9 ± 0.8 4.1 ± 0.8 3.7 ± 0.5  
Urine volume (ml/day) 1.2 ± 0.2 1.0 ± 0.4 1.0 ± 0.3 0.8 ± 0.3 0.008
Osmolality (osmol/l) 2.3 ± 0.4 3.0 ± 0.7 2.9 ± 0.6 4.0 ± 0.9 < 0.001
Urine pH 5.9 ± 0.2 6.2 ± 0.3 6.1 ± 0.4 6.7 ± 0.3 < 0.001
Na (μmol/day) 103 ± 32 94 ± 23 99 ± 35 111 ± 25  
K (μmol/day) 371 ± 109 314 ± 141 384 ± 125 375 ± 92
Ca (μmol/day) 4.6 ± 1.4 1.4 ± 0.6 7.2 ± 2.9 1.8 ± 0.4 < 0.001
Cl (μmol/day) 137 ± 46 139 ± 42 139 ± 47 175 ± 41  
Mg (μmol/day) 30 ± 9 19 ± 12 38 ± 12 31 ± 7 0.009
Pi (μmol/day) 264 ± 34 277 ± 123 257 ± 54 237 ± 46  
Creatinine (μmol/day) 4.7 ± 0.6 4.9 ± 1.8 4.3 ± 0.7 4.2 ± 0.5
Urea (mmol/day) 1.6 ± 0.4 1.6 ± 0.6 1.7 ± 0.5 1.8 ± 0.4
Uric acid (nmol/day) 318 ± 109 639 ± 205 490 ± 147 864 ± 203 < 0.001
Glucose (μmol/day) 2.1 ± 0.4 2.0 ± 0.9 2.3 ± 0.7 2.6 ± 0.4  
Total protein (mg/day) 13 ± 3 13 ± 5 2.5 ± 1.5 2.6 ± 1.9
Albumin (μg/day) 115 ± 26 210 ± 93 86 ± 20 142 ± 53 < 0.001
  1. 28–32-week-old mice were tested for 48 h in metabolic cages. Number per genotype and sex: n = 12. Data are presented as mean ± standard deviation. Significance vs. wild-type controls: Exact P values are indicated for P < 0.05 (2-way ANOVA)
  2. Creatinine urine creatinine analyzed by the enzymatic method