Fig. 1

Compared with WT mice (AC6^+/+), hearts of AC6^ΔN/ΔN and AC6^−/− mice had lower cAMP levels evoked by βAR-stimulation. Total lysates were prepared from the hearts of the indicated mice (2–5 months old). a, b Expression of AC6 and flag-AC6-ΔN was detected by Western blot analysis using anti-AC6 (AC6D, a) and anti-flag (b) antibodies, respectively. Na⁺/K⁺ − ATPase was used as a loading control. c The cAMP level in total lysate evoked by ISO (10 mg/kg, i.p. injection 15 min; AC6^+/+, N = 7; AC6 ^ΔN/ΔN, N = 4; AC6^−/−, N = 3) was measured and normalized to the total amount of protein. The data are presented as the mean ± SEM.^* P < 0.05 vs. AC6^+/+. ^# P < 0.05 vs. the saline group