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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: The type VI adenylyl cyclase protects cardiomyocytes from β-adrenergic stress by a PKA/STAT3-dependent pathway

Fig. 5

The AC6-N regulates the activation of STAT3 in a PKA- dependent manner. a Adult cardiomyocytes were pretreated with or without a PKA inhibitor (KT-5720, 1 μM, 30 min pretreatment) or an Src inhibitor (Src inhibitor 1, 1 μM, 5 min pretreatment) as indicated, followed by a 15- min incubation with isoproterenol (ISO, 10 μM). The scale bar, 10 μm. b The ratios of nuclear STAT3- positive cells in each condition were quantified. Three to four independent experiments were conducted in each condition. At least 30 cardiomyocytes in each condition/experiment were scored. The data are expressed as the mean ± SEM. * P < 0.05 vs. AC6+/+ in each group. # P < 0.05 vs. the basal level in each group. ξ P < 0.05 vs. the ISO-treated cells in each group

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