Fig. 6

Phosphorylation levels of phagocytosis-related kinases in macrophages infected by MARTX_Vv1 domain-deletion mutants. The bacteria were cultured in LB for 4 h, washed, and coincubated with RAW 264.7 cells at MOI 10 for 90 min. Total cell lysate of the infected RAW 264.7 cells was collected, and the phosphorylation level of each kinase was examined by immunoblotting. YJ016: WT strain; HL128: MD mutant. Data are representative of three independent experiments