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Table 1 List of bacterial strains and primers

From: Vibrio vulnificus MARTX cytotoxin causes inactivation of phagocytosis-related signaling molecules in macrophages

Name Description a Note b Reference/Source
Bacterial strain
 YJ016 wild-type strain   [37]
 HL128 MARTXVv1-deficient mutant   [10]
 CJ189 Δu1 mutant   This study
 CJ427 Δrid mutant   This study
 CJ338 Δu2 mutant   This study
 CJ340 Δcpd mutant   This study
 CJ355 Δgd mutant   This study
 CJ433 Δu1Δrid mutant   This study
 CJ190 Δu1Δu2 mutant   This study
 CJ517 ΔridΔu2 mutant   This study
 CJ429 Δu1ΔridΔu2 mutant   This study
 KV105 ΔvvhA mutant   [56]
 KV188 HL128 ΔvvhA mutant   [56]
 CJ525 CJ433 ΔvvhA mutant   This study
 CJ527 CJ529 ΔvvhA mutant   This study
Primer
 DU1F1 5′-TGTCGACGGGTCACAAAGTC-3’ Δu1  
 DU1R1 5′-AGGATCCAGACGCAGTGGTTGGCAC-3’ Δu1  
 DU1F2 5′-AGGATCCGCCGATACGCTGGTTGAGTTG-3’ Δu1  
 DU1R2 5′-TGAGCTCAGAAAGCCCTGCGAAGATCG-3’ Δu1  
 SC01 5′-AGAGCTCCTGGTTGAGTTGGATGTG-3’ Δrid  
 SC02 5′-ATCTAGAACTCGGCTTCCAGATGTA-3’ Δrid  
 SC03 5′-ATCTAGACACACATGGCGACCTAAG-3’ Δrid  
 SC04 5′-AAGCATGCTACCGCTGCTTGCTCTGC-3’ Δrid  
 DF1 5′-TTAGTCGACGTGTTTGGACGCCGACAGAG-3’ Δu2  
 DR1 5′-AGGATCCTTCTACCGCTGCTTGCTCTGC-3’ Δu2  
 DF2f4 5′-AGGATCCGTTGTTGTGACTCCGACAGC-3’ Δu2  
 DR2 5′-TGAGCTCCTGCTGCTCTGATCCAAACC-3’ Δu2  
 SC07 5′-AGAGCTCGGGTTGTTGAGTAAAGCG-3’ Δcpd  
 SC08 5′-ATCTAGAGCCACTCAAACTGTCCTT-3’ Δcpd  
 SC09 5′-ATCTAGAAACGGTATTGCGGAAGGC-3’ Δcpd  
 SC10 5′-AAGCATGCACCCGCTAACTGCCCAAG-3’ Δcpd  
 JL173 5′-TATGAGCTCCAACGCACCCTTCGGTTG-3’ Δgd  
 JL174 5′-GCGTCTAGATAATATTCACCTTCCATT-3’ Δgd  
 JL175 5′-GCGTCTAGACGGCAGGTTAAGCGAGTT-3’ Δgd  
 JL176 5′-TATGAGCTCTTGGTTGAACTGGACTCG-3’ Δgd  
 UVVA0965F 5′-AAATGTCGACACCCACATTAA-3’ ΔvvhA  
 UVVA0965R 5′-AGAGAAAGCTTAAACAGAGTCAT-3’ ΔvvhA  
 DVVA0965F 5′-TCCCAAGCTTCCCACATTACAAC-3’ ΔvvhA  
 DVVA0965R 5′-GGAAGAGCTCACCAAACCCG-3’ ΔvvhA  
 ERM-1 new 5′-AACATATGGATCAAACTCAAGCCCCG-3’ ERM  
 ERM-2 new 5′-AACTCGAGGGTCCCTTTGGCATCATT-3’ ERM  
  1. a u1, rid, u2, cpd and gd correspond to the U1 region, RID domain, U2 region, CPD domain and GD-rich domain, respectively. The underlined sequences in the primers are the restriction sites introduced
  2. bThe MARTXVv1 domain-deletion mutant that the primer was used to isolate or the recombinant peptide that the primer was used to clone