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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Comprehensive comparison of three different animal models for systemic inflammation

Fig. 3

Heme oxygenase-1 expression and activitiy and total fat content in the livers. At the time point indicated, mice were sacrificed and livers were collected for further biochemical and histological analysis. Representative photomicrographs from one of 4–6 different tissue samples stained for HO-1 expression at 0 h, 24 h and 72 h after LPS, PCI or CLP treatment are shown (immunohistochemistry (red-brown color), counterstaining with hematoxylin, original magnification: (a, 400×)). Additionally, HO-1 activities were assessed in the liver 9000 g supernatants of liver homogenates as described in the Materials and Methods section (b). As an approximate measurement for the fat content, the turbidity value in the 9000 g supernatants of the livers was determined (c) and HE stainings were performed. The photomicrograph in (d) shows a representative liver of an endotoxin treated mouse after 24 h, displaying large amounts of fat droplets. Data are given as mean ± standard deviation (SD), n = 4–6 for each group and time point. Statistical significance was determined by using the non-parametric Kruskal-Wallis test, followed by pairwise Mann-Whitney U tests. Statistical comparisons were made versus the control of each group and are denoted as follows: LPS (asterisk, *), PCI (plus, +), CLP (diamond, #). A p value <0.05 (*,+,#) was considered statistically significant; a p value <0.01 (**,++,##) and a p value <0.001 (***,+++,###) are further specified

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