Fig. 3

Novel cage pairing with saline injections or injection-free CPP training failed to prevent LFS-induced hippocampal depotentiation in vitro. a: The schema for a group of mice given saline injections twice a day in a novel big cage for 3 days. Hippocampal slices were prepared on Day 4. b: Time courses of the slope of fEPSPs recorded before, during TBS and LFS, and after LFS in hippocampal slices prepared from mice underwent procedures in A. Inset: Averaged traces of 20 fEPSPs recorded before (a) and 60 min after (b) LFS. c: The schema for mice underwent saline-paired CPP training or i.p. injection-free CPP training. Hippocampal slices were prepared on Day 4. d: Time courses of the slope of fEPSPs recorded before, during TBS and LFS, and after LFS in hippocampal slices prepared from the two groups of mice in c. Inset: Averaged traces of 20 fEPSPs recorded before (a) and 60 min after (b) LFS in each group. e: Bar graphs summarizes the magnitudes of potentiation of fEPSP slope in the three groups of mice in a (Novel cage + saline) and c (i.p.-free CPP and CPP + saline). Depotentiation was induced, analyzed, presented as described in Fig. 1. *p < 0.05 vs. 100%, paired t-test. ^# p < 0.05 vs. the novel cage + saline group and ^& p < 0.05 vs. the CPP group (one-way ANOVA with Newman-Keuls post-hoc analysis)