Fig. 4

Pretreatment with TCS1102, a dual orexin receptor antagonist, restored LFS-induced hippocampal de-potentiation in vitro in mice underwent saline-paired CPP training. a: The schema for mice given vehicle (Veh) or TCS1102 (TCS, 20 mg/kg, i.p.) 30 min prior daily saline-paired CPP training process. Hippocampal slices were prepared on Day 4. b: Time courses of the slope of fEPSPs recorded before, during TBS and LFS, and after LFS in hippocampal slices prepared from the two groups in a. Inset: Averaged traces of 20 fEPSPs recorded before (a) and 60 min after (b) LFS in each group. c: Bar charts summarize the magnitudes of potentiation of fEPSP slope in vehicle- or TCS1102-treated mice. Depotentiation was induced, analyzed, presented as described in Fig. 1. *p < 0.05 vs. 100%, paired t-test. ^# p < 0.05 vs. the TCS group, un-paired t-test