Fig. 3

TNFSF ligands mRNA expressions in murine ischemic renal injury. IRI was induced in C57Bl6 mice as described in methods. a Representative images of renal sections stained with PAS or for F4/80 and CD3. Original magnification: ×100. The tubular injury, as well as F4/80 and CD3 positive area per hpf, were quantified. Data represents mean ± s.e.m. *p < 0.05, **p < 0.01, *p < 0.001. b Quantitative RTPCR was performed on cDNAs derived from the injured kidneys. The mRNA expression levels were normalised to 18 s rRNA expression level. The heatmap represents the relative expression of mRNA levels at day 1, 5 and 10 versus control. c The statistical analysis of TNFSF ligands mRNA expression levels in the injured kidneys at day 1, 5 and 10 versus control kidneys is represented using volcano graphs. P < 0.05 is considered statistically significant. Dotted line represent p = 0.05