Fig. 4

TNFSF ligands mRNA expressions in murine oxalate-induced CKD. Oxalate-induced CKD was induced in mice as described in methods. a Representative images of renal sections stained with PAS or for F4/80, CD3 and collagen1. Original magnification: ×100. The tubular injury, F4/80, CD3 positive cells, and collagen-1 were quantified. Data represents mean ± s.e.m. *p < 0.05, **p < 0.01, *p < 0.001. b Quantitative RTPCR was performed on cDNAs derived from the kidneys of mice on day 7, 14 and 21. The mRNA expression levels were normalised to 18 s rRNA expression level. The heatmap represents the relative expression of TNFSF ligands mRNA levels at day 7, 14 and 21versus day 0. c The statistical analysis of TNFSF ligands mRNA expression levels in the kidneys at day 7, 14 and 21 versus day 0 is represented using volcano graphs. P < 0.05 is considered statistically significant. Dotted line represent p = 0.05