Fig. 5

TNFSF ligands mRNA expressions in murine autologous anti-GBM nephritis. Autologous anti-GBM nephritis was induced in mice as described in methods. a Representative images of renal sections stained with PAS or for F4/80 and CD3. Original magnification: ×100. The glomerulosclerosis, F4/80, and CD3 positive cells were quantified. Data represents mean ± s.e.m. *p < 0.05, **p < 0.01, *p < 0.001. b Quantitative RTPCR was performed on cDNAs derived from the kidneys of mice on day 0, 14, 21 and 42. TNFSF ligands mRNA expression levels were normalised to 18 s rRNA expression levels. The heatmap represents the relative expression of TNFSF ligands mRNA levels at day 0, 14, 21 and 42 versus day 0. c The statistical analysis of TNFSF ligands mRNA expression levels in the kidneys at day 5, 10 and 35 versus control is represented using volcano graphs. P < 0.05 is considered statistically significant. Dotted line represent p = 0.05