Fig. 1
AA treatment alters the cellular redox balance at the onset of differentiation. a Changes in the intracellular redox state measured by flow cytometry in proliferating cells. Cells were treated with 5 μM PMA (positive control). b Intracellular redox state was assessed up to 24 h after the differentiation was induced. Data are compared between untreated differentiating cells and differentiating cells treated with 200 μM AA only up to 24 h post-differentiation. c Changes in intracellular redox state measured in untreated differentiating cells were compared with values obtained for differentiating cells treated with 5 mM NAC only up to 24 h post-differentiation. n = ~ 3.104 cells per time point. Values are mean ± SD of three independent experiments. *P ≤ 0.05 compared with untreated differentiating cells at t = 0 h; # P ≤ 0.05 between each treatment condition at each time point; ns, non-significant