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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/β-catenin/ROS signaling dependent manner

Fig. 3

AA treatment promotes human NPC neurogenesis. a Confocal images of neurons (TUBB3-positive cells: green) and glial cells (GFAP-positive cells: red) after 72 h of differentiation. The representative image panel in the figure was selected according to a comparable cell number for each condition (n = 350 to 400 cells per microscopic field). Scale: 50 μm. b Neurons were counted and the neuronal yield was then calculated (% of the population). Data for untreated differentiated cells were compared with cells treated for two periods of incubation with 200 μM AA or 5 mM NAC (short treatment vs. full treatment), or with 15 mM LiCl or 0.5 μM RuR. n = ~ 3000 cells per condition. Values are mean ± SD of three independent experiments. *P ≤ 0.05 compared with untreated differentiated cells at t = 72 h; # P ≤ 0.05 between short treatment and full treatment for each condition; ns, non-significant

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