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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/β-catenin/ROS signaling dependent manner

Fig. 4

AA treatment does not affect the cell viability. a Cell viability was monitored at 0 h, 24 h and 48 h of differentiation. Cells were seeded at the same concentration prior to any treatment or the induction of differentiation. Prior to the differentiation was initiated, cells were pre-treated for 1 h with 15 mM LiCl or 0.5 μM RuR, and for 24 h with 200 μM AA or 5 mM NAC. A further exposure of the cells with each reagent was performed from the induction of the differentiation: up to the 24 h post-differentiation (i.e. short treatment) for LiCl, AA and NAC, and up to 2 h for RuR. Nuclei were then stained as the nuclear labelling reports the number of cells in the population. Confocal images of nuclei were acquired, and nuclei were further counted to determine the remaining cell density and to calculate the cell viability. n = ~ 500 cells per time point and condition. Values are mean ± SD of three independent experiments. *P ≤ 0.05 compared with untreated differentiating cells at t = 0 h. b Cell viability was assessed in proliferating or 72 h-differentiated cells for various treatments. Proliferating cells were treated for 24 h with 200 μM AA. Alternatively, differentiation was induced up to 72 h, concomitantly with distinct drug treatments: 15 mM LiCl, 200 μM AA, 5 mM NAC or 0.5 μM RuR for two periods of incubation (short treatment vs. full treatment). n = ~ 3000 cells per condition. Values are mean ± SD of three independent experiments. *P ≤ 0.05 compared with untreated proliferating cells

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