Fig. 6

AA treatment stimulates the expression of the WNT/β-catenin signaling target gene MYC. MYC mRNA levels (fold change) were evaluated by quantitative real-time PCR at 0, 24 and 48 h after the differentiation was induced. Data for untreated cells were compared with cells treated with 15 mM LiCl, 200 μM AA (short treatment) or 0.5 μM RuR. Values are mean ± SD of three independent experiments. *P ≤ 0.05 compared with untreated differentiating cells at t = 0 h; ^# P ≤ 0.05 between each treatment condition at t = 48 h; ns, non-significant