Fig. 7
Elevated ROS metabolism mediated by AA treatment facilitates DVL2-NXN complex dissociation. a Representative pseudocolor images of FRETeff between DVL2 and NXN proteins obtained at 0 h, 0.5 h, 1 h and 3 h after the differentiation was initiated. Images were acquired in untreated differentiating cells and compared with proliferating cells treated with 1 mM H2O2, and with differentiating cells treated with 200 μM AA or 0.5 μM RuR. The colour scale at the right of the panel is shown in %. Scale: 10 μm. b FRETeff values were averaged and compared between untreated- and H2O2-treated proliferating cells. c Mean values of FRETeff were also calculated at 0 h, 0.5 h, 1 h, 2 h, 3 h and 4 h of differentiation for untreated- and AA- or RuR-treated differentiating cells (n = ~ 50 cells per time point and condition). Values are means (% of FRETeff) ± SD of three independent experiments. *P ≤ 0.05 compared with untreated differentiating cells at t = 0 h; # P ≤ 0.05 each treatment condition at each time point; ns, non-significant