Fig. 5

High expression of c-Myc in polyps of Apc ^Min/+ Ticam1 ^−/− mice. a Gene expression in polyps prepared from 20 to 25-weeks-old mice were quantified by RT-qPCR. More than 3 mice in each group were used. **; p < 0.01 in Student’s t-test. b Protein was extracted from pooled three polyps in distal small intestine and separated by SDS-PAGE. Expression levels of c-Myc protein were detected by Western blotting. GAPDH is used as an internal control. Each lane shows results from pooled samples prepared from individual mice. Lane 1-2: polyps from Apc ^Min/+ mice, Lane 3-5: polyps from Apc ^Min/+ Ticam-1 ^−/− mice. c Immune staining of polyps in small intestine prepared from 20 to 25-weeks-old Apc ^Min/+ Ticam1 ^−/− mice using anti-c-Myc and anti-EpCAM antibodies. Data show representative results of two independent experiments