Skip to main content
Fig. 5 | Journal of Biomedical Science

Fig. 5

From: The TLR3/TICAM-1 signal constitutively controls spontaneous polyposis through suppression of c-Myc in Apc Min/+ mice

Fig. 5

High expression of c-Myc in polyps of Apc Min/+ Ticam1 /− mice. a Gene expression in polyps prepared from 20 to 25-weeks-old mice were quantified by RT-qPCR. More than 3 mice in each group were used. **; p < 0.01 in Student’s t-test. b Protein was extracted from pooled three polyps in distal small intestine and separated by SDS-PAGE. Expression levels of c-Myc protein were detected by Western blotting. GAPDH is used as an internal control. Each lane shows results from pooled samples prepared from individual mice. Lane 1-2: polyps from Apc Min/+ mice, Lane 3-5: polyps from Apc Min/+ Ticam-1 −/− mice. c Immune staining of polyps in small intestine prepared from 20 to 25-weeks-old Apc Min/+ Ticam1 /− mice using anti-c-Myc and anti-EpCAM antibodies. Data show representative results of two independent experiments

Back to article page