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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Lipopolysaccharide pretreatment increases protease-activated receptor-2 expression and monocyte chemoattractant protein-1 secretion in vascular endothelial cells

Fig. 3

Effects of trypsin on ERK activation. Upper panel: Typical blot showing ERK phosphorylation in EA. hy926 cells treated with trypsin. EA. hy926 cells (1 × 106/mL) were treated with 5 μg/mL of trypsin for the indicated duration, and p-ERK levels were determined through Western blotting as described and normalized to the total ERK levels. Lower panel: Normalization of the p-ERK and total ERK levels. Bar graphs represent means ± SEM from four independent experiments. *p < 0.05 compared with the control group

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