Fig. 4

Enhancement effects of LPS pretreatment on MAPK phosphorylation in EA. hy926 cells treated with trypsin. EA. hy926 cells were treated in the absence or presence of LPS (0.25, 0.5, 1, and 2 μg/mL) for 24 h and with 5 μg/mL of trypsin for 10 min. a Representative data of the p-ERK, total ERK, p38, and JNK MAPK levels. b Normalization of the p-ERK and total ERK levels. c Normalization of the p-p38 and total p38 levels. d Normalization of the p-JNK and total JNK levels. Bar graphs represent means ± SEM from four independent experiments. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group