Fig. 5

Enhancement effects of LPS pretreatment on MCP-1 secretion and cell adhesion functions in EA. hy926 cells treated with trypsin. EA. hy926 cells were treated in the absence or presence of LPS (0.25, 0.5, 1, and 2 μg/mL) for 24 h, followed by treatment with 5 μg/mL of trypsin for 12 h. a MCP-1 protein levels were quantified through immunoblotting, and β-actin was used as the loading control. The upper panel shows a typical blot. Bar graphs represent means ± SEM from four independent experiments. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group b Effects of LPS pretreatment on trypsin-induced mononuclear cell–EC adhesion. ECs were treated with the indicated LPS concentrations (0, 0.25, 0.5, 1, and 2 μg/mL) for 24 h, followed by treatment with trypsin (5 μg/mL) for 12 h. The adhesion assay with mononuclear cells was performed in sextuplicate. Microphotographs (100×) show the adhesion of mononuclear cells to the trypsin-stimulated ECs treated with the indicated LPS concentrations