Fig. 1

Evolutionary history of selected amino acid sequences of ZIKV and DENV NS5 proteins. The tree shows that NS5 sequences from Brazilian ZIKV isolates grouped with those from Asian ZIKV lineage. The evolutionary history was inferred by using the Maximum Likelihood method based on the JTT matrix-based model [1]. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 195 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 896 positions in the final dataset. Evolutionary analyses were conducted in MEGA7