Table 1 Specific primers were designed for DNA sequencing of coding regions in the beta-globin gene. Besides, the primers were designed to genotyping of IVSII-74 (rs7480526) by the ARMS-PCR methods
| Primer name | Primer Sequence | PCR size (bp) | Annealing temperature |
|---|---|---|---|
| HBB 1F | GTA GCA ATT TGT ACT GAT GGT ATG G | ||
| HBB 2R | CTT CCA CAC TGA TGC AAT CAT TC | 861 | 63° |
| HBB 3F | ATG TAT CAT GCC TCT TTG CAC C | ||
| HBB 3R | GCA CTG ACC TCC CAC ATT CC | 578 | 63° |
| IVSII-74 N | TTA AGT TCA TGT CAT AGG AAG GTG AG | ||
| IVSII-74 M | TTA AGT TCA TGT CAT AGG AAG GTG AT | ||
| IVSII-74 R | ATC ACT GTT ATT CTT TAG AAT GGT GC | 589 | 60° |
| GAPDH F | CAT GGC CTC CAA GGA GTA AG | ||
| GAPDH R | GGT TGA GCA CAG GGT ACT TTA | 219 | 60° |