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Table 1 Specific primers were designed for DNA sequencing of coding regions in the beta-globin gene. Besides, the primers were designed to genotyping of IVSII-74 (rs7480526) by the ARMS-PCR methods

From: Haplotypes inside the beta-globin gene: use as new biomarkers for beta-thalassemia prenatal diagnosis in north of Iran

Primer name Primer Sequence PCR size (bp) Annealing temperature
HBB 1F GTA GCA ATT TGT ACT GAT GGT ATG G   
HBB 2R CTT CCA CAC TGA TGC AAT CAT TC 861 63°
HBB 3F ATG TAT CAT GCC TCT TTG CAC C   
HBB 3R GCA CTG ACC TCC CAC ATT CC 578 63°
IVSII-74 N TTA AGT TCA TGT CAT AGG AAG GTG AG   
IVSII-74 M TTA AGT TCA TGT CAT AGG AAG GTG AT   
IVSII-74 R ATC ACT GTT ATT CTT TAG AAT GGT GC 589 60°
GAPDH F CAT GGC CTC CAA GGA GTA AG   
GAPDH R GGT TGA GCA CAG GGT ACT TTA 219 60°