Fig. 1

Relative Id-1 expression in NSCLC clinical samples, and its clinical significance. a Relative mRNA levels of Id-1 in NSCLC tissues and in paired noncancerous tissues. Id-1 expression was examined by qPCR and normalized to GAPDH expression. ^** P < 0.01, compared with non-tumor counterpart. b Western blot of Id-1 protein quantification in 4 NSCLC tissues and in paired noncancerous tissues. β-Actin was a loading control. c Representative images of Id-1 staining in 96 paired NSCLC tissues. Scale bar, 50 μm. d Kaplan-Meier curves for overall survival of two groups defined by high and low expression of Id-1 in patients with NSCLC. ^** p = 0.008