Fig. 2

Id-1 was associated with viability and mobility features of NSCLC cell. a Determination of Id-1 expression levels in four NSCLC cell lines and the immortalized normal human bronchial epithelial cell line (BEAS-2B). The efficiency of Id-1 silencing and overexpression in NSCLC cell lines was measured by Western blot. β-Actin was a loading control. b and c Representative results for cell proliferation rate were evaluated in Id-1-knockdown (b) or Id-1-overexpressing (c) NSCLC cells by using CCK-8 assay. ^* p < 0.05, ^** p < 0.01. d and e, Representative images (left) and quantification (right) of the clone formation assays are shown in Id-1-knockdown (d) or Id-1-overexpressing (e) NSCLC cells. ^* p < 0.05, compared with control groups. F and G, Representative results (right) and Quantification (left) of the migration and invasion showing the effect of Id-1 knockdown (f) or Id-1 overexpression (g) on the migratory abilities of NSCLC cells. ^* p < 0.05. h Western blot analysis of the phenotypic markers, including E-cadherin and N-cadherin in Id-1 silencing cells. β-Actin was used as the loading control. i Confocal microscopy analysis of the E-cadherin and N-cadherin. The red and green signal represents the staining of the corresponding protein, and the blue signal represents the nuclear DNA staining by DAPI