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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Id-1 promotes migration and invasion of non-small cell lung cancer cells through activating NF-κB signaling pathway

Fig. 3

Id-1 positively regulate IκB phosphorylation and NF-κB activation in NSCLC cells. a, Western blotting showing total and phosphorylated IκBα in Id-1-silenced H226 cells and Id-1-overexpressing A549 cells. β-Actin was a loading control. b, Western blotting showing total and phosphorylated IκBα in NSCLC tissues. β-Actin was a loading control. c, Scatter plots showing the positive linear correlation between the Id-1 expression and phosphorylation level of IκBα in NSCLC tissues. d, Western blot for nuclear and cytoplasm p65 in Id-1-overexpressing A549 cells. β-Actin and Histone 3 (H3) is the loading control for cytoplasm and nuclear, respectively. e, Western blot for Id-1, nuclear and cytoplasm p65 in Id-1- silenced H226 cells after incubation with TNF-α (10 ng/ml) for 24 h. β-Actin and Histone 3 (H3) is the loading control for cytoplasm and nuclear, respectively. f, Graphic presentation of the relative abundances (fold induction versus the controls).* P < 0.05 versus control groups after TNF-α treatment

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