Fig. 2

OSS induces sustained activations of β1 and β3 integrins in ECs plated on FN, but only transient activations of these integrins in ECs plated on LM. ECs plated on FN (a and b) or LM (c and d) were kept under static conditions (C) or subjected to OSS (0.5 ± 4 dyn/cm²) for 1/6 h (10 min), 1/2 h (30 min), 1 h, 4 h, and 24 h; Western blot analysis was used to determine the activations of the β1 and β3 integrins by using HUTS-4 and LIBS-2 antibodies, respectively. Data are means ± SEM from three independent experiments. ^*P < 0.05 vs. static control cells