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Table 1 Primer sequences for RT-qPCR

From: MicroRNA-365 alleviates morphine analgesic tolerance via the inactivation of the ERK/CREB signaling pathway by negatively targeting β-arrestin2

Primer

Forward (5′-3′)

Reverse (5′-3′)

miR-365

CGTAATGCCCCTAAAAAT

GTGCAGGGTCCGAGGT

U6

CTCGCTTCGGCAGCACA

AACGCTTCACGAATTTGCGT

β-arrestin2

CCAGGGTCTTCAAGAAGTC

TTGCCCAAGTACACGGT

ERK

CTCTGTCATTGCCACCA

ATCCACTCTCCATCTCCAT

CREB

TACCCAGGGAGGAGCAATACA

GGTGCTGTGCGAATCTGGTAT

GAPDH

CCGAGGGCCCACTAAAGG

TGCTGTTGAAGTCACAGGAGACA

  1. RT-qPCR Reverse transcription quantitative polymerase chain reaction, ERK Extracellular signal-regulated kinase, CREB cAMP-response element binding protein, GAPDH Glyceraldehyde-3-phosphate dehydrogenase
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Journal of Biomedical Science

ISSN: 1423-0127