Fig. 2

Kinetics of CB533 activity, and ROS assay in A375 cells. a Cells were treated with 5 μM CB533 and cell viability was determined at indicated time points. 4 ± 0.5 h after treatment number of viable cells was reduced by 50%. The experiment was performed three times, and a representative experiment is shown. Data are expressed as the mean ± SD of triplicate samples. P ≤ 0.05 compared to untreated control cells (by Student’s t test analysis). b Cells were treated for 4 h with 5 μM CB533, 1,4-NQ or DMSO control. Production of ROS is only observed in 1,4-NQ-treated cells. A representative experiment is shown