Fig. 1

BRET assay monitoring interaction of HO-1 or HO-2 with CPR. a The constructs of Luc-fused HO and CPR-fused GFP proteins. b HEK 293 T cells were co-transfected with the constant amount of Luc-HO-1 or Luc-HO-2 construct and increasing amounts of GFP or CPR-GFP construct as indicated. After 24 h, cells were reseeded in triplicate in 96-well plates for 18 h. Luciferase substrate was then added into each well and the BRET signal was monitored and expressed as the ratio of signals detected at 515 nm/410 nm. Data shown are mean ± SE of three independent experiments