Skip to main content
Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Identification of danthron as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity

Fig. 4

Danthron interferes the physical interaction of HO-1 but not HO-2 with CPR. Cell lysate was prepared from HEK293T cells transfected with CPR-GFP construct for 24 h. CPR-GFP protein was then immuno-precipitated by GFP-antibody-conjugated resin, followed by incubation with His-HO-1 or HO-2 protein in the absence or presence of 10 μM indicated compounds at room temperature for 1 h with rotation. The HO protein pulled down by CPR-GFP was then examined by Western blot analysis

Back to article page

Journal of Biomedical Science

ISSN: 1423-0127