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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: The chondroitin sulfate moiety mediates thrombomodulin-enhanced adhesion and migration of vascular smooth muscle cells

Fig. 1

Thrombomodulin (TM) expression, glycosylation, and localization in HASMCs and A7r5 transfected with TM cDNA constructs. a HASMCs were serum-starved for 48 h (Lane 1) and then stimulated with PDGF-BB (10 ng/ml) for 6 h without (Lane 2) or with (Lane 3) ChABC (0.5 U/ml) treatment for 1 h. b A7r5 cells were transfected with pEGFP (Lane 1), pEGFP-TM (Lane 2) or pEGFP-TMS490,492A (Lane 4). The cells overexpressing TM were treated with ChABC before harvest (Lane 3). Western blotting was performed with antibodies specific to either human TM (Top) or β-actin (Bottom). c The localization of TM in VSMCs. a and b, HASMCs were serum-starved for 36 h and treated without (a) or with (b) PDGF-BB for 6 h. c, d, and e, A7r5 cells transfected with pEGFP (c), pEGFP-TM (d) or pEGFP- TMS490,492A (e) were fixed, processed for TM immunofluorescence (a and b), and examined under a confocal microscope. Scale bar: 50 μm

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