Fig. 5

Thrombomodulin (TM) expression increased FAK phosphorylation in A7r5 cells upon adhesion. a A7r5 cells transfected with pEGFP, pEGFP-TM, or pEGFP-TM^S490,492A were detached and seeded to type I collagen-coated cover slip for 3 h. Cells were processed for immunofluorescence of FAK^Y397 phosphorylation, stained with DAPI, and observed under a confocal microscope. Scale bar: 20 μm. b and c A7r5 cells, stably expressing TM-GFP, TM^{S490, 492A}-GFP or GFP, were cultured to confluence. Following detachment, cells were resuspended and attached to type I collagen-coated dish for 3 h. Cell lysates were analyzed for FAK phosphorylation and expression. b shows a representative result of immunoblotting; (c) summarizes the quantitative results of FAK phosphorylation ratio and FAK expression normalized by α-tubulin levels expressed as mean ± SEM (n = 3)