Fig. 6
Thrombomodulin expression did not affect PDGF-stimulated proliferation of A7r5 cells. A7r5 cells were transfected with pEGFP, pEGFP-TM, or pEGFP-TMS490,492A, serum-starved for 48 h, and treated with PDGF-BB for 24 h. Cells were fixed and stained with DAPI (blue) and Ki-67(red). a shows representative results; (b) summarizes the ratio of Ki-67-positive cells; (c) summarizes total cell numbers in cells expressing vector alone, TM-GFP, and TMS490, 492A-GFP from at least 3 independent experiments. Results were expressed as mean ± SEM. Scale bar: 100 μm