Fig. 8

Apoptosis analysis of RSV-infected N2a neuronal cells. After RSV infection, N2a cells were stained with FITC, Annexin-V and PI and subjected to flow cytometry analysis. Panels a, b, c, d, e, f, g, h, and i represent the apoptotic statuses of N2a cells at 0 h, 1 h, 2 h, 4 h, 12 h, 24 h, 2 d, 3 d and 4 d after RSV infection, respectively. Panels j, k, and l represent the apoptotic statuses of uninfected N2a cells at 2 d, 3 d and 4 d, respectively. Data are expressed as the mean ± SEM, and all tests were run in triplicate (shown in fig. m). ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 indicate the P values of early apoptosis compared with those of the uninfected control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 indicate the P values of late apoptosis compared with those of the uninfected control group